MOESM2 of DUF3669, a “domain of unknown function” within ZNF746 and ZNF777, oligomerizes and contributes to transcriptional repression

Additional file 2. Negative control luciferase reporter assays in HeLa cells and the HAP1 cell system (wildtype, TRIM28 knockout and SETDB1 knockout cells, respectively) using Gal4 fusion constructs with segments of human PRDM9 (P9/24–97, covering the KRAB-like domain; P9/24–363, covering the extended N-terminal KRAB-SSXRD-PRSET part; see reference [48]). Assay procedure as described in legends of Figs. 3 and 4. Bar plots represent normalized mean repression factor values ± STDEV of four (HeLa) or six (HAP1 system) biological replicates from two (HeLa) or three (HAP1) independent experiments relative to experiment-specific values for the Gal4-DBD alone.