MOESM2 of Concurrent interactome and metabolome analysis reveals role of AKT1 in central carbon metabolism

Additional file 2: Figure S1. Detailed workflow to identify interacting partners of AKT1 followed by their subsequent analysis. Figure S2. Filters employed to generate the final list of AKT1 Interactors. Figure S3. KEGG pathway enrichment for AKT1 binding partners using WebGestalt. Figure S4. Validating the effect of AKT1 inhibitor (MK-2206) on AKT1 activity by western blotting. Figure S5. Specimen depiction of relative incorporation of labeled carbon in G6P and FBP metabolites in MK-2206 treated and untreated samples. Figure S6. Possible labeling pattern for key metabolite upon feeding cells with 13C6 Glucose. Figure S7. Specimen chromatograms depicting estimated false discovery rates in Lys C and Trypsin digested MK-2206 treated (Lys8Arg10) and untreated (Lys0Arg0) samples. Figure S8. Specimen chromatograms showing metabolite peaks in the untreated and MK-2206 treated samples for five different metabolites at one of the targeted time points. Figure S9. Chromatograms are corresponding to ion spectra of palmitic acid (as standard) and free fatty acids. Figure S10. Schematic diagram of the CCM pathways depicting uptake of glucose by the cells and its subsequent utilization across different metabolites. Proteins showing perturbed association with AKT1 inhibition and their corresponding reaction steps are highlighted in red colour in figure.