12936_2017_1959_MOESM2_ESM.docx (19.79 kB)
MOESM2 of Concentration of Plasmodium falciparum gametocytes in whole blood samples by magnetic cell sorting enhances parasite infection rates in mosquito feeding assays
journal contribution
posted on 2017-08-05, 05:00 authored by Isaie Reuling, Will Stone, Marga de Vegte-Bolmer, Geert-Jan Gemert, Rianne Siebelink-Stoter, Wouter Graumans, Kjerstin Lanke, Teun Bousema, Robert SauerweinAdditional file 2: Figure S2. Effect of different flow-rate MACS on gametocyte infectivity. Number of oocysts per mosquito after membrane-feeding assay (MIDI cages). 1 600 µL of gametocyte-infected whole blood from starting material, fed directly. 2 9000 µL of gametocyte-infected whole blood from starting material added once over MACS column with 23G needle, eluted and reconstituted in 600 µL of uninfected blood before feed. 3 9000 µL of gametocyte-infected whole blood from starting material added once over MACS column with 21G needle, eluted and reconstituted in 600 µL of uninfected blood before feed. Vertical bars indicate mean with SEM.