12977_2019_489_MOESM2_ESM.tif (200.05 kB)
MOESM2 of Characterization of resistance to a potent d-peptide HIV entry inhibitor
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posted on 2019-10-23, 08:28 authored by Amanda Smith, Matthew Weinstock, Amanda Siglin, Frank Whitby, J. Francis, Christopher Hill, Debra Eckert, Michael Root, Michael KayAdditional file 2. SPR sensorgrams for PIE12 monomer binding to IZN36 WT (left panel) or Q577R (right panel), processed in Scrubber2 (BioLogic Software) and used for the equilibrium fit shown in Fig. 2. Six threefold dilutions of PIE12 monomer (617 nM to 2.54 nM) were flowed over the WT surface, and ten threefold dilutions (50 µM to 2.54 nM) were flowed over the Q577R surface. The calculated KD’s are 0.031 µM for WT and 2.0 µM for Q577R.
