MOESM2 of Characterization of nAChRs in Nematostella vectensis supports neuronal and non-neuronal roles in the cnidarian–bilaterian common ancestor

Additional file 2: Figure S2. Phylogeny generated in IQ tree using an edited alignment that includes the transmembrane domains and double cysteines. IQ-tree generated maximum likelihood phylogeny determining the relationship of potential cnidarian acetylcholine receptors to the known bilaterian nicotinic and muscarinic acetylcholine receptors. The GABA receptors, indicated by a purple box, served as the out-group for our analysis. Muscarinic receptors are indicated by a green box. The ultrafast bootstrap values for critical nodes of interest are written in red. Tree generated using alignment in Additional file 10: Table S3. Heat map also indicates ultrafast bootstrap values. N. vectensis sequences are in red text on tips of tree branches.