MOESM2 of CHIP functions as an oncogene by promoting colorectal cancer metastasis via activation of MAPK and AKT signaling and suppression of E-cadherin

Additional file 2. CHIP expression in individual colorectal cancer cell lines. (a) qRT-PCR analysis of the mRNA expression of CHIP in HT-29, DLD-1, COLO320DM, and CaCO2 cell lines. β-actin normalized gene expression was displayed as an internal control. Independent experiments were displayed at least three times. Error bars were calculated as SD of the mean. (b) Western blotting analysis of the protein expression level of CHIP in the whole cell extract of HT-29, DLD-1, COLO320DM, and CaCO2 cell lines. Actin was used as a loading control.