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MOESM2 of A novel non-sulphamoylated 2-methoxyestradiol derivative causes detachment of breast cancer cells by rapid disassembly of focal adhesions

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posted on 2018-11-19, 05:00 authored by Mandie Botes, Tamarin Jurgens, Zohreh Riahi, Michelle Visagie, Rustelle Janse van Vuuren, Anna Joubert, Iman Bout
Additional file 2: Figure S2. EE-15-one related cell detachment in MDA-MB-231 cells is not due to induced cell death. Medium from MDA-MB-231 cells exposed to 5 μM EE-15-one was collected at the indicated times and analysed for LDH activity. The graph shows the percentage LDH activity as compared to the positive control. The average result of three independent experiments was plotted with error bars representing s.e.m. *Indicates significant differences between negative control medium and medium from EE-15-one treated cells as calculated by student’s t-test at a P < 0.05.

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