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MOESM2 of A lipidated peptide of Mycobacterium tuberculosis resuscitates the protective efficacy of BCG vaccine by evoking memory T cell immunity
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posted on 2017-10-06, 05:00 authored by Pradeep Rai, Sathi Chodisetti, Weiguang Zeng, Sajid Nadeem, Sudeep Maurya, Susanta Pahari, Ashok Janmeja, David Jackson, Javed AgrewalaAdditional file 2: Figure S2. Gating procedure for monitoring the expression of CXCR3 + CCR6 on IFN-Îł + IL-17A expressing CD4 T cells. The P1 gate was made on lymphocyte zone and P2 gate on SSC-A and CD4+ T cells. The display of IL-17A+IFN-Îł (P3 gate) was monitored on P2 zone (CD4+ and SSC+). The expression of CXCR3+CCR6 was examined on P3 region (IL-17A+IFN-Îł positive cells). The unstained cells failed to show any CD4+ T cell population.