MOESM1 of l-Arabinose triggers its own uptake via induction of the arabinose-specific Gal2p transporter in an industrial Saccharomyces cerevisiae strain
2018-08-23T05:00:00Z (GMT) by
Additional file 1: Table S1. Specific primer sequences designed for GAL1, GAL2, GAL5/PGM2, GAL7, GAL10, HXT9, HXT10 and SGA1 which are used in RT-qPCR. Table S2. Comparative RNA-Seq analysis results of genes related to the central carbon metabolism of S. cerevisiae DS58227 (wild-type), DS61143 (intermediate strain) and DS61180 (l-arabinose consuming strain). (a) S. cerevisiae DS61180 (l-arabinose consuming strain) grown on l-arabinose compared to growth of the same strain on d-glucose as reference. (b) S. cerevisiae DS58227 (wild-type) compared to DS61180 (l-arabinose consuming strain) as reference, both grown on d-glucose. (c) S. cerevisiae DS61143 (precursor strain of DS61180) compared to DS61180 (l-arabinose consuming strain) as reference, both grown on d-glucose. Genes are assigned to classes that are involved in the redox (I) (genes encoding NAD(P)H dependent proteins), the energy (II) (genes encoding ATP dependent proteins) and the galactose metabolism (III), as well as to a NAD(P)H and ATP independent (IV) class. Samples were analyzed as biological triplicates taken during mid exponential growth of the three strains on the individual substrates d-glucose and/or l-arabinose. Regulation R is indicated as symbols: (â) up-regulated, (-) un-regulated, (â) down-regulated compared to the reference condition that is underlined in the label. E indicates the error of log2FC values.