12934_2015_360_MOESM1_ESM.pdf (188.58 kB)
MOESM1 of Lactobacillus plantarum displaying CCL3 chemokine in fusion with HIV-1 Gag derived antigen causes increased recruitment of T cells
journal contribution
posted on 2015-10-22, 05:00 authored by Katarzyna Kuczkowska, Geir Mathiesen, Vincent Eijsink, Inger ØynebråtenAdditional file 1. This file shows the influence of L. plantarum on increasing (a) and reduction (b) of the amount of migrated cells. (A) Migration of Esb-MP cells towards RPMI-1640 medium alone and RPMI-1640 medium containing L. plantarum harboring pEV (Lp_Ev). (B) Migration of Esb-MP cells towards RPMI-1640 medium supplemented with 3 ng/ml CCL3, and towards RPMI-1640 medium containing L. plantarum harboring pEV and supplemented with 3 ng/ml CCL3. The total number of migrated cells was counted by flow cytometry. The presented data are from one representative experiment. The experiment was performed at least three independent times, with similar results.