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MOESM1 of Urinary alpha-1 antitrypsin and CD59 glycoprotein predict albuminuria development in hypertensive patients under chronic renin-angiotensin system suppression

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posted on 2016-01-16, 05:00 authored by Laura Gonzalez-Calero, Marta Martin-Lorenzo, Fernando Cuesta, Aroa Maroto, Montserrat Baldan-Martin, Gema Ruiz-Hurtado, Helena Pulido-Olmo, Julian Segura, Maria Barderas, Luis Ruilope, Fernando Vivanco, Gloria Alvarez-Llamas
Additional file 1. Figure S1. Representative image of 2D-DIGE gel. Figure S2. Principal component analysis (PCA) graph. Each dot represents a urine sample used in the discovery phase (DIGE analysis). Table S1. Baseline medication for those patients including in the discovery phase. Data are expressed as percentages (%). ACEi: angiotensin converting enzyme inhibitors; ARB: angiotensin receptor blockers. N: normoalbuminuria; dnHA: de novo high albuminuria; MHA: maintained high albuminuria. Table S2. Baseline medication for those patients including in the validation phase. Data are expressed as percentages (%). ACEi: angiotensin converting enzyme inhibitors; ARB: angiotensin receptor blockers. N: normoalbuminuria; dnHA: de novo high albuminuria; MHA: maintained high albuminuria. Table S3. Proteins identifiedper gel spot with significant alteration (one-way ANOVA). The table shows the number of unique peptides identified, % sequence coverage and trends observed for each protein between compared groups (increase or decrease in the group located in the upper part of the ratio). Two spots contain a mixture of two proteins each, thus changes in expression forthose spots cannot be attributed to any of the two proteins initially. When one protein was identified in several spots, observed variations between groups followed the same trend (e.g. CD59 and alpha-1-antitrypsin). Table S4. SRM-LCMS/MS analysis conditions for those proteins confirmed in the validation phase with statistical signification (ANOVA <0.0001). Details of protein transitions (precursor and fragments masses), collision energy and peptide sequences are included.

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