MOESM1 of Transcriptomic and proteomic responses to very low CO2 suggest multiple carbon concentrating mechanisms in Nannochloropsis oceanica

Additional file 1: Figure S1. A schematic diagram of the experimental design. Nannochloropsis oceanica cells were firstly grown to logarithmic phase under air enriched with 5% CO2. After adaption to new environment under 5% CO2 for an hour, the cells were then cultured under either 100 pm or 50,000 ppm CO2 concentration. Samples were collected at 0, 3, 6, 12 and 24 h from each condition (three biological replicate columns for each) by syringe for physiological characterization and multi-omics (transcriptome, proteome and metabolome) profiling.