MOESM1 of Towards the understanding of the enzymatic cleavage of polyisoprene by the dihaem-dioxygenase RoxA

Additional file 1: Table S1. Effect of potential external haem ligands like imidazole and related low molecular compounds on activity and on UVvis-properties of RoxA-Wt as isolated. Figure S1. RoxA incubated with ferricyanide (left) or pyrogallol (right). Figure S2. UVvis spectra of RoxA. Figure S3. EPR spectra of RoxA. Figure S4. RoxA incubated with different haem ligands. Figure S5. Part of the RoxA active site. Figure S6. Comparison of UVvis sprectra of RoxA Wt and RoxA-F317A. Figure S7. Reaction of RoxA-Wt and RoxA-F317Y with carbon monoxide. Figure S8. (left) Effect of pyridine and imidazole on the activity of RoxAF301Y. Figure S9. EPR spectra of RoxA. Figure S10. Optical spectrum of RoxA after reduction and reoxidation under anaerobic conditions (enlarged on the right). Figure S11. EPR spectra of RoxA-Wt in the presence of small substrate analogues.