Springer Nature
Browse
12915_2019_718_MOESM1_ESM.pdf (3.63 MB)

MOESM1 of Temperature-dependent regulation of upstream open reading frame translation in S. cerevisiae

Download (3.63 MB)
journal contribution
posted on 2019-12-07, 04:30 authored by Shardul Kulkarni, Fujun Zhou, Neelam Sen, Hongen Zhang, Alan Hinnebusch, Jon Lorsch
Additional file 1: Figure S1. β-galactosidase activity assay to calculate usage of NCC start site at multiple growth temperature. Figure S2. Temperature dependent alterations in usage of NCC start site are not evoked due to changes in the levels of initiation factors. Figure S3. Ribosome profiling at multiple growth temperatures. Figure S4. Changes in growth temperature lead to changes in translation of uORFs. Figure S5. Analysis of translation efficiency changes of conserved uORFs upon changes in growth temperature. Figure S6. Analysis of the properties of various sets of uORFs and the mRNAs harboring them. Figure S7. Analysis of uORFs showing highest changes in translation at 20 °C and 37 °C. Figure S8. Boxplot analyses of 5’-UTR features for the most highly regulated NCC uORFs. Figure S9. Analysis of TE-changes of mORFs. Figure S10. Wiggle track images of examples of mRNAs that show temperature-dependent changes in translation of one or more uORF and the mORF. Figure S11. uORF-mediated temperature-dependent regulation of AGA1 mRNA.

Funding

National Institutes of Health

History