MOESM1 of Systematic unravelling of the inulin hydrolase from Bacillus amyloliquefaciens for efficient conversion of inulin to poly-(Îł-glutamic acid)

Additional file 1: Figure S1. Alignment of the amino acid sequences of CscA and other sucrose hydrolase and invertase. Figure S2. Product analysis of CscA with inulin and sucrose substrate. 50 g/L of inulin solution was incubated with 3 mg of purified CscA per mL at 55°C, pH 7.5 for 5 h. (a) Preparative hydrolysis of inulin with purified CscA enzyme by HPLC analysis; (b) hydrolysis of sucrose with purified CscA enzyme by HPLC analysis.