MOESM1 of Shoot tip culture: a step towards 13C metabolite flux analysis of sink leaf metabolism

Additional file 1: Figure S1. Mass isotopomer distribution (%) of isopulegone in each leaf pair at 30 μmol m-2 s-1 light intensity. (a) Shoot-tip culture was initiated with one developing leaf pair and after 15 days of culture, total four leaf pairs (older than 6 days) were observed. (b) Shoot-tip culture started without leaves and after 15 DALV, total three leaf pairs (older than 6 days) were observed (mean ± standard error, n = 5). Figure S2. Mass isotopomer distribution (%) of menthofuran in each leaf pair at 30 μmol m-2 s-1 light intensity. (a) Shoot-tip culture initiated with one developing leaf pair and after 15 days of culture, total four leaf pairs (older than 6 days) were observed. (b) Shoot-tip culture started without leaves and after 15 DALV, total three leaf pairs (older than 6 days) were observed (mean ± standard error, n = 5). Figure S3. Photographs of shoot-tip culture (a) Shoot-tip culture at 15 DALV under 5 μmol m-2 s-1 light intensity. (b) Shoot-tip culture at 20 DALV under 5 μmol m-2 s-1 light intensity (c) Overview of shoot- tip culture system inside growth chamber. Two and three leaf pairs (older than 6 days) were observed at 15 and 20 DALV, respectively. Figure S4. Mass isotopomer distribution (%) of pulegone in control shoot-tips, nurtured with unlabelled glucose as sole carbon source. Calculated label dilution in this control shoot-tips was 99.74% (after natural abundance correction) with negligible amounts of 13C (0.26%) was considered as an experimental or measurement error (mean ± standard error, n = 5). This result remained virtually similar in control treatments within leaves of different age grown under different light intensities. Table S1. Consumption of glucose (mg) by shoot-tip culture. Glucose consumption was measured after 15 DALV growth of the shoot-tip culture under different light intensities (mean ± standard error, n = 5). Table S2. Production of photosynthetic pigments and volatile terpenes in 15 days old leaves from in vivo plants. Leaves were collected from the peppermint plant grown under natural condition (mean ± standard error, n = 5). Table S3. Photosynthetic assimilation rates of 15 days old leaves from shoot-tip cultures and normal plants. The measurements were taken using the LI-6400XT portable gas exchange system (Li-Cor Inc., Lincoln, NE, USA) (mean ± standard error, n = 5). Table S4. Details of measured amino acids and their fragments. Table S5. Label dilution (%) into monoterpene in two leaf pairs of oregano shoot-tip culture after 15 DALV under 10 μmol m-2 s-1 light intensity. Labelled U-13C glucose (2%) was supplemented in the basal media (mean ± standard error, n = 5).