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MOESM1 of RUNX1 mutations promote leukemogenesis of myeloid malignancies in ASXL1-mutated leukemia

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posted on 2019-10-23, 08:25 authored by Rabindranath Bera, Ming-Chun Chiu, Ying-Jung Huang, Tung-Huei Lin, Ming-Chung Kuo, Lee-Yung Shih
Additional file 1: Table S1. List of qRT-PCR primer sets to check mRNA expression of different genes. Figure S1. Simultaneous expression of ASXL1-R693X and RUNX1-R135T augmented cell proliferation. Figure S2. Co-expression of ASXL1-R693X and RUNX1-R135T in U937 cells and transformed cells had no effect on CD11b expression in the presence of DMSO. Figure S3. ASXL1-mutant K562 cell line expressed H3K27me3 and endogenous ASXL1 in K562 cells interacted with endogenous EZH2 protein, and K562 transformed cells had no effect on CD61 expression in the presence of DMSO. Figure S4. ASXL1-R693X collaborates with RUNX1-R135T in myeloid transformation. Figure S5. Collaborative mutations of ASXL1-R693X and RUNX1-R135T enhanced ID1 expression. Figure S6. RUNX1 and HIF-1α do not interacted with ASXL1. Figure S7. Stability of RUNX1-R135T mutant protein is more than RUNX1-WT protein. Figure S8. Collaboration of RUNX1-R135T with ASXL1-R693X in the augmentation of HIF-1α and its target gene.

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National Science Council

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