MOESM1 of RNA polymerase II depletion promotes transcription of alternative mRNA species

Additional file 1: Figure S1. Confirmation of RPB2 Northern blot data by qRT-PCR. The schematic in panel A indicates locations of the primers that amplify the long RPB2 mRNA (purple; amplifies only the 4297 nt long form) and the short RPB2 mRNA (green; amplifies both 4010 nt and 4297 nt forms). Panel B presents the RT- PCR analysis of RNA isolated from the RPB1-FRB strain following nuclear depletion (+RAP). RNA levels were normalized to RNA from an intergenic region on chromosome V as done in Figure 5.