MOESM1 of Production of a human milk oligosaccharide 2′-fucosyllactose by metabolically engineered Saccharomyces cerevisiae

Additional file 1: Figure S1. Comparison of the volumetric concentrations of extracellular and intracellular 2-FL in a 125-mL flask, produced by fed-batch fermentation of the engineered S. cerevisiae D452-2_LFF strain after 30 and 36 h. All data points are the means of experimental data from duplicate fed-batch fermentations. S. cerevisiae D452-2_LFF indicates S. cerevisiae D452-2 harboring fkp encoding L-fucokinase/guanosine 5’-diphosphate-L-fucose phosphorylase, fucT2 encoding α-1,2-fucosyltransferase, and LAC12 encoding lactose permease.