MOESM1 of Probing the subcutaneous absorption of a PEGylated FUD peptide nanomedicine via in vivo fluorescence imaging

Additional file 1: Fig. S1. Overlay of ion exchange chromatograms showing the separation of singly sulfo-Cy5 labeled mFUD and 10-40 kDa PEG-mFUD from the unreacted and multiply labeled peptides. The collected fraction containing the singly labeled drug is indicated with arrows. An anionic exchanger in conjunction with 20 mM Tris (pH 8) A side and 1 M NaCl in 20 mM Tris Buffer (pH 8) B side mobile phases were used to elute the peptides.