13104_2019_4697_MOESM1_ESM.xlsx (22.73 kB)
MOESM1 of Physiological phenotyping of mammalian cell lines by enzymatic activity fingerprinting of key carbohydrate metabolic enzymes: a pilot and feasibility study
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posted on 2019-10-23, 08:27 authored by Julian Geiger, Rebecca Doelker, Sofia Salรถ, Thomas Roitsch, Louise DalgaardAdditional file 1. Enzyme activity assay excel template: excel file for enzyme activity assay setup support. The file contains a blank layout sheet for test sample and control sample distribution on a 96-well plate. Additional sheets contain master mix volume calculations for activity assays for Aldolase, Glucose-6-phosphate Dehydrogenase, Hexokinase, Phosphofructokinase, Phosphoglucose isomerase, and Phosphoglucosemutase. Adjustment of the desired number of control and test samples (grey fields) results in recalculation of required volumes. All sheets are printable on A4 paper and can be filled out digitally or manually. https://doi.org/10.6084/m9.figshare.7859801 .