MOESM1 of Phospholipase C inhibits apoptosis of porcine primary granulosa cells cultured in vitro

Additional file 1: Figure S1. Effect of U73122 on the mRNA abundance of PLCB1 in porcine granulosa cells. Cells were challenged with the doses of U73122(from 0 μM to 5 μM) for the times given. Data are mean ± S.E.M.of three independent replicates. For each treatment, means without common letters are significantly different(p<0.05). Figure S2. Effect of m3M3FBS on the mRNA abundance of PLC in porcine granulosa cells. Cells were challenged with the doses of m3M3FBS (from 0 μM to 50 μM) for the times given. Data are mean ± S.E.M.of three independent replicates. For each treatment, means without common letters are significantly different(p<0.05).Figure S3. One representative scatter diagram at each time point for apoptosis induced by U73122 measured with annexin V/PI staining in porcine granulosa cells. Cells were challenged with 0.5 μM U73122 for 4 h (gene) or for the times given(percentage of apoptotic cells), which were processed for annexin V/PI staining and measured by flow cytometry assay. Figure S4. One representative scatter diagram at each time point for apoptosis induced by m3M3FBS measured with annexin V/PI staining in porcine granulosa cells. Cells were challenged with 0.5 μM m3M3FBS for 4 h(gene) or for the times given(percentage of apoptotic cells), which were processed for annexin V/PI staining and measured by flow cytometry assay. Figure S5. Fluorescence intensity of intracellular Ca2+ in porcine granulosa cells. The area in the histogram represents the fluorescence intensity variation of granulosa cells measured by Flow cytometry and analyzed by FlowJo V10. A and B indicate the fluorescence intensity treated with DMF (Control) and U73122; C and D indicate the fluorescence intensity cultured with DMSO (Control) and m-3M3FBS.