MOESM1 of Peroxiredoxin 1 induces inflammatory cytokine response and predicts outcome of cardiogenic shock patients necessitating extracorporeal membrane oxygenation: an observational cohort study and translational approach

Additional file 1: Figure S1. CD14 and TLR4 expressions on CD14+ monocytes analyzed by flow cytometry. (A) A representative forward (FSC) and side scattering (SSC) plot of lymphocytes (R1), monocytes (R2) and granulocytes (R3) was shown. (B) Isotype control antibody was used to establish the background fluorescence levels of monocytes (M1), and the percentage of positive cells was determined after staining of the cells with specific antibody (M2). (C) The CD14+ monocytes population was gated as in the SSC/CD14 dot plot. The CD14 (D) and TLR4 (E) expression levels were calculated as mean fluorescence intensity (MFI) of the populations as gated using the methodology described for panel B.