MOESM1 of PLDα1-knockdown soybean seeds display higher unsaturated glycerolipid contents and seed vigor in high temperature and humidity environments

Additional file 1: Figure S1. Schematic procedure for construction of soybean GmPLDα1RNAi vector for plant transformation. Figure S2. Phylogenetic analysis of PLD genes from the soybean genome and their expression patterns. Figure S3. Mechanism of plant resistant to stress and gene expression of GmPLDαs and stress-related genes under high temperature and humidity condition in comparison to under normal conditions. Figure S4. Analyses of lipids in PLDα1KD and wild-type developing seeds under different growth conditions. Figure S5. Phylogenetic analysis of FAD genes from the soybean genome and their expression patterns. Figure S6. Phylogenic analysis of Acyl-CoA:lysophosphatic acid acyltransferase (LPAAT) genes and their expression profiles. Figure S7. Phylogenic analysis of phosphatidic acid hydrolase (PAH) genes and their expression profiles. Figure S8. Phylogenic analysis of acyl-CoA:diacylglycerol acyltransferase (DGAT) genes and their expression profiles. Figure S9. Phylogenic analysis of phospholipid:diacylglycerol acyltransferase (PDAT) genes and their expression profiles. Figure S10. Phylogenic analysis of choline/ethylamine kinase (CEK) genes and their expression profiles. Figure S11. Phylogenic analysis of CTP: phosphocholine cytidylyltransferase (CCT) genes and the expression profiles. Figure S12. Phylogenic analysis of diacylglycerol:cholinephosphotransferase (DAG-CPT or AAAT) genes and their expression profiles. Figure S13. Phylogenic analysis of phosphatidylcholine:diacylglycerol cholinephosphotransferase (PDCT) genes and their expression profiles. Figure S14. Phylogenetic analysis of PLA genes from the soybean genome and their expression patterns. Figure S15. Phylogenic analysis of 2-lysophosphatidylcholine acyltransferase (LPCAT) genes and their expression profiles.