MOESM1 of Mutational Mtc6p attenuates autophagy and improves secretory expression of heterologous proteins in Kluyveromyces marxianus

Additional file 1: Figure S1. Strategies for screening desirable mutants in a high-throughput manner. Figure S2. A linear relationship was existed between the activity of Est1E and the enzyme concentration. Figure S3. Identification of the mutants with excellent ability in expressing the heterologous Est1E. Figure S4. Purification and quantification of the Est1E expressed and secreted by the T1/Est1E recombinant. Figure S5. Establishment of the specific mutants using CRISPR/Cas9 system. Table S1. Amino-acid sequences of the Est1E and the full or truncated Mtc6p. Table S2. Primers and DNA sequences used in this study. Table S3. Information of the specific SNP or indel in the genome of T1 mutant.