MOESM1 of Microbial network for waste activated sludge cascade utilization in an integrated system of microbial electrolysis and anaerobic fermentation

Additional file 1. Table S1. The main contents of sludge fermentative liquid on the 3rd day, after different pretreatments. Table S2. The main characteristics of raw waste activated sludge. Table S3. The detected number of sequences, OTUs and diversity. Fig. S1. Particle size distribution in sludge, obtained through different pretreatments. Control: sludge without treatment; A: Alkaline; F: Freeze/thaw; U: Ultrasonic treatment. Fig. S2. Lysis ratio of increased SCOD to TCOD in sludge, after different pretreatments. Fig. S3. MEC reactor setup: COD removal and Coulombic efficiency obtained by feeding artificial wastewater. Fig. S4. VFAs content in different sludge fermentative liquids. Fig. S5. Main contents of COD removal (Polysaccharide, protein, VFAs) in MECs fed with different sludge fermentative liquids. Fig. S6. Current change in the last three batch MECs fed with different sludge fermentative liquids. Fig. S7. Rarefaction curves (a) and Shannon diversity (b) base on pyrosequencing of bacterial communities. The OTUs were defined by 3 % distance. Fig. S8. Taxonomic classification of bacterial communities of sludge fermentative liquid at the phylum (a), class (b) and genus (c) levels. Relative abundance was defined as the number of sequences per sample. Fig. S9. The setup of single chamber microbial electrolysis cell (MEC). Fig. S10. A flow schematic representation of experimental methodology and reactor setup.