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MOESM1 of Microbial community dynamics and coexistence in a sulfide-driven phototrophic bloom

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posted on 2020-01-18, 04:45 authored by Srijak Bhatnagar, Elise Cowley, Sebastian Kopf, Sherlynette Pérez Castro, Sean Kearney, Scott Dawson, Kurt Hanselmann, S. Ruff
Additional file 1: Supplementary Materials, Methods and Results. Figure S1. Natural blooms in Trunk River. Figure S2. Color and appearance of samples from all holes, depths, and timepoints. Figure S3. Filters that were used for biomass measurements and spectral analysis. Figure S4. Total cell count of three samples (A2, A7 and K7). Figure S5. Depth profile representation of chemical data presented in Fig. 2. Figure S6. Physicochemistry. Iron, nitrate, ammonium, acetate, Ca2+, and K+ measurements. Figure S7. Individual diversity indices of all samples. Figure S8. Trajectories of community structure in hole A, E and K. Figure S9. Relative sequence abundance of the 20 most abundant clades on phylum, class, order, family and genus level, as well as the 20 most sequence abundant ASVs (amplicon sequence variants). Figure S10. Relative sequence abundance of Chlorobiales ASVs. Figure S11. Relative change of ASV abundance between surface (V1) and deeper layers (V2-4). Figure S12. Chlorobiales phylogeny. Figure S13. Circular map of metagenome-assembled genomes (MAGs). Figure S14. Chlorobiales phylogenomics. Figure S15. Protein comparison of Bin 6. Figure S16. Protein comparison of Bin 10. Figure S17. Genes involved in sulfur cycling. Figure S18. CRISPR arrays and cas genes predictions Bin 6. Figure S19. CRISPR arrays and cas genes predictions Bin 10. Figure S20. Relative sequence abundance of viral family-level clades. Table S1. Overview of sequencing output and diversity indices. Table S2. Genome statistics. Table S3. Average nucleotide identity (ANI) comparisons. Table S4. Oxidative phosphorylation and chlorophyll biosynthesis genes of Bin 6 and Bin 10. Table S5. CRISPR-Cas system information for each metagenome-assembled genome.

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