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MOESM1 of Metabolic engineering of Acremonium chrysogenum for improving cephalosporin C production independent of methionine stimulation

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posted on 2018-06-07, 05:00 authored by Jiajia Liu, Wenyan Gao, Yuanyuan Pan, Gang Liu
Additional file 1: Table S1. Strains and plasmids used in this study. Table S2. Primers used in this study. Fig. S1. Cephalosporin C production of WT detected by UPLC/MS in the MDFA medium with or without addition of 3.2 g/L methionine. Fig. S2. Mycelium dry weight of A. chrysogenum in the MDFA medium with or without addition of 3.2 g/L methionine. Fig. S3. Sequence alignment and phylogenetic analysis of the SAM synthetase family proteins. Fig. S4. Cephalosporin C production of WT and WT/pAg1PT-G418 in the MDFA medium with or without addition of 3.2 g/L methionine. Fig. S5. Construction and validation of the AcsamS overexpressed strain (AcsamsOE). Fig. S6. Cephalosporin C production of WT and AcsamsOE was detected by UPLC/MS in MDFA medium. Fig. S7. Mycelium dry weight of AcsamsOE in the MDFA medium with or without addition of 3.2 g/L methionine. Fig. S8. Cephalosporin C production of AcsamsOE in the MDFA medium supplemented with different concentration of SAM. Fig. S9. Sequence alignment of the leucine carboxyl methyltransferase superfamily proteins. Fig. S10. Construction and validation of the Acppm1 disruption mutant (Acppm1DM). Fig. S11. Cephalosporin C production of WT and Acppm1DM was detected by UPLC/MS. Fig. S12. Mycelium dry weight of Acppm1DM, Acppm1CM, Acppm1OE in the MDFA medium with or without addition of 0.32 g/L methionine. Fig. S13. The relative transcriptional level of AcsamS in WT and Acppm1DM. Fig. S14. The relative transcriptional level of AcmetH, AccysD, AcmecA and mecB of WT in the MDFA medium with or without addition of 3.2 g/L methionine. Fig. S15. Cephalosporin C production of Acppm1DM and Acppm1DM-AcsamsOE. Fig. S16. The relative transcriptional level of mecB in Acppm1DM. Fig. S17. Construction and validation of the mecB overexpressed strain (Acppm1DM-mecBOE). Fig. S18. Cephalosporin C production of WT and Acppm1DM-mecBOE was detected by UPLC/MS in MDFA medium. Fig. S19. Mycelium dry weight of WT and Acppm1DM-mecBOE in the MDFA medium supplemented with 0, 0.32 g/L and 3.2 g/L of methionine respectively. Fig. S20. Construction and validation of Acppm1DM-mecBOE-AcsamsOE. Fig. S21. Cephalosporin C production of Acppm1DM-mecBOE-AcsamsOE.

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National Natural Science Foundation of China

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