MOESM1 of MEIS2C and MEIS2D promote tumor progression via Wnt/β-catenin and hippo/YAP signaling in hepatocellular carcinoma

Additional file 1 : Figure S1. (A) siMEIS2 efficiency measured by western blot in HEK293T cells. (B) Relative MEIS2C/D mRNA levels were measured by q RT-PCR in different cell lines. Figure S2. (A) Three predicted MEIS2 binding sites detected by ChIP in HEK293T cells. Input DNA were used as positive controls. Immuno-precipitated DNA by anti-IgG antibody was used as negative controls. PCR was used to find out the MEIS2D and PBX1 binding site for miR-1307 gene. (B) Results of ChIP of M2 sites DNA fragments performed with Flag-MEIS2A/B/C. (C) Results of ChIP of three predicted MEIS2 binding sites DNA fragments performed with Flag-MEIS2B. Figure S3. (A) HCC-LM3 cells were transfected with indicate siRNA, miRNA or controls. Then, immunoblotting was performed to examine SHP2. (B) YAP intracellular distribution detected by immunofluorescence staining. (C, D) HCC-LM3 cells were transfected with indicated plasmids, siRNAs and controls. Then, Immunoblotting and immunofluorescence staining were performed to examine LATS1 and YAP expression and localization. Figure S4. (A) MHCC97H cells were transfected with indicated siRNA and vectors. YAP localization investigated by immunofluorescence staining. (B) Lysates were detected by indicated antibody. HCC-LM3 and MHCC97H cells were transfected with siMEIS2 and indicated rescue vectors. Then, proliferation function of MEIS2C or MEIS2D was measured by CCK-8 Kit(C). Relative YAP and β-catenin mRNA levels were measured by q RT-PCR after 48 h (D). Figure S5. RNA expression correlation between MEIS2A/B and genes were investigated in HCC patients. Table S1. Correlations between clinic pathological features and relative MEIS2C/D expression. Table S2. Predicted putative MEIS2 binding site sequence and position on miR-1307 Promoter (3 KB) by using JASPAR ( jaspar.genereg.net )