sorry, we can't preview this file
13046_2019_1474_MOESM1_ESM.eps (5.76 MB)
MOESM1 of Long non-coding RNA PAXIP1-AS1 facilitates cell invasion and angiogenesis of glioma by recruiting transcription factor ETS1 to upregulate KIF14 expression
figure
posted on 2019-12-11, 10:44 authored by Haiyang Xu, Guifang Zhao, Yu Zhang, Hong Jiang, Weiyao Wang, Donghai Zhao, Hongquan Yu, Ling QiAdditional file 1: Figure S1. LncRNA PAXIP1-AS1 affects glioma cell migration, invasion, and angiogenesis by regulating KIF14 expression. A, Transwell assay to examine the migration ability of glioma cells (200 ×). B, Transwell assay to examine the invasive ability of glioma cells (200 ×). C, The tube formation assay to examine angiogenesis of HUVECs (100 ×). D, Western blot analysis for expression of E-Cadherin, N-Cadherin, MMP-2, VEGF-A, ETS1 and KIF14 normalized to GAPDH in glioma cells. * p < 0.05 vs. the oe-NC + sh-NC group, # p < 0.05 vs. the oe-PAXIP1-AS1 + oe-ETS1 + sh-NC group. The values are measurement data and expressed as mean ± standard deviation. Comparisons among multiple groups were made by one-way ANOVA with Tukey’s post hoc test. Cell experiment was repeated three times independently.