MOESM1 of Increasing n-butanol production with Saccharomyces cerevisiae by optimizing acetyl-CoA synthesis, NADH levels and trans-2-enoyl-CoA reductase expression

2016-11-25T05:00:00Z (GMT) by Virginia Schadeweg Eckhard Boles
Additional file 1: Figure S1. Metabolic pathway for n-butanol production via reverse β-oxidation in yeast. Figure S2. Comparison of glucose consumption and final OD600 of n-butanol production strains. Figure S3. Comparison of ethanol concentration of n-butanol production strains. Figure S4. Enzymatic reactions of adhE from E.coli. Figure S5. Stability assay of adhEA267T/E568K/R577S. Figure S6. n-Butanol production of VSY13 under aerobic conditions with addition of pantothenate. Figure S7. Aerobic fermentation of VSY15 containing NADH oxidase nox from Streptococcus pneumoniae. Table S1 Relevant primers for this study. Table S2. Yeast promoters and terminators used for the expression of n-butanol pathway genes and endogenous pantothenate synthesis in this study. Table S3. Statistical analysis of n-butanol production.