MOESM1 of Increased ETV4 expression correlates with estrogen-enhanced proliferation and invasiveness of cholangiocarcinoma cells

Additional file 1: Table S1. Primer sequences for gene expression analysis of ERs. Figure S1. Relative expression of ERs in CCA cells compared to MCF-7 and MDA-MB-231 breast cancer cells: (A) ER-ι; (B) ER-β. Figure S2. Efficacy of shETV4 plasmid transfection and knockdown: (A) Transfection efficacy determined by expression of GFP and visualized under fluorescence inverted microscope with 40x original magnification, cell type and conditions were labelled in the picture; (B) Knockdown efficacy in transfected cells determined by RT-real time PCR and compared to parental cell mRNA.