MOESM1 of Improving plant transient expression through the rational design of synthetic 5′ and 3′ untranslated regions

Additional file 1. Sequence of the Synth expression cassette. The different elements follow the same colour scheme as in Fig. 1: blue for the 35S promoter and nos terminator, red for the 5S0 5′UTR, green for the cloning site, and yellow for the 3S0 3′UTR. Restriction sites indicated in Fig. 1 are underlined in the sequence: Pac1 at the 5′ end, BsmB1 in 5S0, Bsa1 in the cloning site, Sap1 in 3S0, and Asc1 at the 3′ end.