MOESM1 of Improving plant transient expression through the rational design of synthetic 5′ and 3′ untranslated regions
2019-09-19T04:09:02Z (GMT) by
Additional file 1. Sequence of the Synth expression cassette. The different elements follow the same colour scheme as in Fig. 1: blue for the 35S promoter and nos terminator, red for the 5S0 5′UTR, green for the cloning site, and yellow for the 3S0 3′UTR. Restriction sites indicated in Fig. 1 are underlined in the sequence: Pac1 at the 5′ end, BsmB1 in 5S0, Bsa1 in the cloning site, Sap1 in 3S0, and Asc1 at the 3′ end.