MOESM1 of HTLV-1 CTCF-binding site is dispensable for in vitro immortalization and persistent infection in vivo

Additional file 1: Fig. S1. Ablation of HTLV-1 CTCF-binding site does not qualitatively decrease HTLV-1-specific antibody response. The HTLV antibody response was assessed qualitatively at 0, 4, and 12 weeks post-inoculation in a representative rabbit from each condition via a modified MP Diagnostics HTLV Blot 2.4 Western Blot Assay protocol (MP Biomedicals LLC, Santa Ana, CA). The supplied alkaline phosphatase conjugated goat anti-human immunoglobulin gamma (IgG) was substituted for an alkaline phosphatase conjugated goat anti-rabbit IgG (ab6722; Abcam, Cambridge, United Kingdom). Plasma from each condition was diluted 1:10. Reactive HTLV-1 proteins are labeled on the left. rgp46-1 (HTLV-1-specific recombinant envelope surface protein); p53 (Gag precursor); p24 (capsid protein); p19 (matrix protein); GD21 (recombinant transmembrane envelope protein). “Serum control” denotes serum immunoglobulin levels among.