MOESM1 of HOXA repression is mediated by nucleoporin Nup93 assisted by its interactors Nup188 and Nup205
2016-12-03T05:00:00Z (GMT) by
Additional file 1: Figure S1. a Co-Immunoprecipitation of Nup93 upon depletion of Nup205, shows reduced interaction between Nup93 and Nup188. b Nup93 ChIP PCR experiment was performed in Nup188 and Nup205 depleted cells, a representative gel image from 2 independent biological replicates. c ChIP qPCR for control PanH3 in siNeg, Nup188 and Nup205Kd cells, Y-axis represents immunoprecipitated DNA relative to 1% input (N = 2, independent biological replicates). d qRT-PCR analyses was performed upon Nup93, Nup188 and Nup205 depletion to check their relative transcript levels. Y-axis indicates fold change in levels of mRNA normalized to untreated cells. Error bars: S.E.M, data from a single experiment that includes 3 technical replicates. e qRT-PCR analyses upon Nup93 over expression in a background of Nup188 or Nup205 depletion as indicated. Y-axis indicates fold change in levels of mRNA normalized to untreated cells. Error bars: S.E.M, data from two biological replicates that includes 6 technical replicates. f Western blot quantification of Fig. 2e from 3 independent biological replicates. Y-axis: relative band intensity quantified using Image-J. g Ponceaue staining for western blot represented in Fig. 2e.