MOESM1 of From one to all: self-assembled theranostic nanoparticles for tumor-targeted imaging and programmed photoactive therapy

Additional file 1: Figure S1. Chemical structures and synthetic route of hyaluronic acid-cysteamine dihydrochloride-cholesterol (HSC) conjugates. Figure S2. FT-IR of different conjugates including cysteamine dihydrochloride-cholesterol and hyaluronic acid-cysteamine dihydrochloride-cholesterol (HSC) conjugates. Figure S3. Stability evaluation of HSCI NPs via sizes (A) and zeta potentials measured by DLS. Figure S4. UV curves of DPBF (ROS detection probe) under the laser irradiation of HSCI NPs as a function of time. Figure S5. Plot of temperature change as a function of different concentrations of HSCI NPs over 10 min under laser irradiation. Figure S6. Cell viability of different incubation groups consisting of free IR780 (A) and HSCI NPs (B). Figure S7. Confocal microscopy images of control group in which the cells were incubated with saline for 0.5 h, the lysosome was stained with Lyso-Tracker Green. Figure S8. (A) PA signal spectrum of HSCI NPs at various concentrations. (B) Linear relationship between signal intensity and concentration. (C) MSOT imaging phantoms including different concentration of HSCI NPs embedded in agar gel cylinders. Figure S9. MSOT imaging at different times of mice treated with free IR780. Figure S10. Quantification of MSOT imaging signal at tumor site at different times of mice treated with free IR780 and HSCI NPs. Figure S11. Fluorescence imaging at different times of mice treated with free IR780.