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MOESM1 of FGF-induced LHX9 regulates the progression and metastasis of osteosarcoma via FRS2/TGF-β/β-catenin pathway
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posted on 2019-11-26, 04:29 authored by Shuang-Qing Li, Chao Tu, Lu Wan, Rui-Qi Chen, Zhi-Xi Duan, Xiao-Lei Ren, Zhi-Hong LiAdditional file 1: Figure S1. The relative expression levels of LHX1–9 and knockdown efficiencies of shLHX9, shbeta-catenin and shFRS2 in OS cells. (A) The relative expression levels of LHX1–9 were measured by qRT-PCR in hFOB1.19 and U2OS. LHX1–9 mRNA levels were normalized to GAPDH mRNA (n = 3). (B) U2OS cells or Saos-2 cells were un-transfected or transfected with negative control shRNA (shNC) or shRNA targeting LHX9 (shLHX9), beta-catenin and FRS2, respectively, then they were cultured for 48 h. The expression of LHX9, beta-catenin and FRS2 in blank control cells, shNC- or shLHX9-transfected cells was measured by Western blot. GAPDH was used as the loading control.