MOESM1 of FGF-induced LHX9 regulates the progression and metastasis of osteosarcoma via FRS2/TGF-β/β-catenin pathway

Additional file 1: Figure S1. The relative expression levels of LHX1–9 and knockdown efficiencies of shLHX9, shbeta-catenin and shFRS2 in OS cells. (A) The relative expression levels of LHX1–9 were measured by qRT-PCR in hFOB1.19 and U2OS. LHX1–9 mRNA levels were normalized to GAPDH mRNA (n = 3). (B) U2OS cells or Saos-2 cells were un-transfected or transfected with negative control shRNA (shNC) or shRNA targeting LHX9 (shLHX9), beta-catenin and FRS2, respectively, then they were cultured for 48 h. The expression of LHX9, beta-catenin and FRS2 in blank control cells, shNC- or shLHX9-transfected cells was measured by Western blot. GAPDH was used as the loading control.