MOESM1 of Extraction of amplifiable DNA from embalmed human cadaver tissue

2017-12-13T05:00:00Z (GMT) by Lindsay Gielda Stefanie Rigg
Additional file 1: Table S1. Variables tested in Method Development. Quantification (ng/Îźl), absorbance 260/280 ratio, and DNA electrophoresis gel description of DNA isolated from 4 cadaver donor grey matter cerebellar tissue. Each experiment as performed in duplicate and mean values are indicated. Development of the proposed method included testing of the indicated variables; PBS washes, incubation temperature, and proteinase K treatment. The final method was determined based on a combination of high quantity and quality DNA, yielding large molecular weight fragments.