MOESM1 of Expression of immunogenic structural proteins of cyprinid herpesvirus 3 in vitro assessed using immunofluorescence
2016-01-08T05:00:00Z (GMT) by
Additional file 1. Graphical representation of fluorescence from MAbs 20F10 and 10A9 recognising Koi herpesvirus antigens over the course of infection in KF-1 cells in 96 well microtitre plates. Graphs are of cells analysed during the first day of infection. (A) DAPI fluorescence of cells; (B) Difference of relative FITC to DAPI stain from blank wells at 0 hpi. Red line = MAb 20F10 (Capsid-associated antigen); Blue line = MAb 10A9 (Envelope glycoprotein antigen) Mean Âą SE (n = 4 individual cell cultures).