MOESM1 of Expressing accessory proteins in cellulolytic Yarrowia lipolytica to improve the conversion yield of recalcitrant cellulose
2017-12-11T05:00:00Z (GMT) by
Additional file 1: Figure S1. PCR verification of Y. lipolytica transformants expressing multiple cellulases and accessory proteins (A) YLC7, Lane 1 to 6: BGL1, BGL2, 4UASTrEGI, TrEGII, 4UASNcCBHI, 4UASTrCBHII; (B) YLC8, Lane 1 to 6: BGL1, BGL2, 4UASTrEGI, TrEGII, 4UASNcCBHI, 4UASTrCBHII, TrXYNII; (C) YLC9, Lane 1 to 7: BGL1, BGL2, 4UASTrEGI, TrEGII, 4UASNcCBHI, 4UASTrCBHII, TrLPMOA; (D) YLC10, Lane 1 to 7: BGL1, BGL2, 4UASTrEGI, TrEGII, 4UASNcCBHI, 4UASTrCBHII, TrSWO1; (D) YLC11, Lane 1 to 8: BGL1, BGL2, 4UASTrEGI, TrEGII, 4UASNcCBHI, 4UASTrCBHII, TrXYNII, TrLPMOA. Figure S2. Western blot analysis of the heterologous rhTrEGI protein secreted by the engineered Y. lipolytica strains: lane 1, Endo H-treated secretome of YLC6b (20 μL); lane 2, Endo H-treated secretome of YLC7b (20 μL). Figure S3. Characterization of the recombinant XYNII expressed in Y. lipolytica. (a) Effect of pH on the activity of rhXYNII; (B) Effect of temperature on the activity of rhXYNII. Figure S4. Screening of Y. lipolytica expressing cellulases and accessory enzymes on YNB indication plate containing supplemented with 0.2% w/vAzo-CM-Cellulose. Lane 1, Y. lipolytica control; Lane 2 to 4, YLC8, YLC9 and YLC10. Figure S5. The growth of Y. lipolytica in defined medium containing 10 g/L gluconic acid or 10 g/L glucose. Table S1. The sequences of the oligonucleotide primers used for PCR verification of Y. lipolytica-transformants. Table S2. Comparison of cellulose utilization and biomass yield of cellulolytic Y. lipolytica grown on different cellulosic substrates for 120 h in aerobic cultivation without the addition of ascorbic acid.
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