MOESM1 of Experimental and bioinformatics study for production of l-asparaginase from Bacillus licheniformis: a promising enzyme for medical application

Additional file 1: Table S1. Levels of reaction conditions of process parameters as independent variables studied in RSM experimental design for optimization of l-asparaginase production by the selected test mutant. Table S2. Experiments that were deduced by the RSM experimental design and performed for l-asparaginase production by the mutant. Table S3. Pairwise distances among l-asparaginases of bacterial species presented in the phylogenetic tree shown in Fig. 1. Table S4. Pairwise distances among l-asparaginases of Bacillus licheniformis, E. coli and Erwinia chrysanthemi presented in the phylogenetic tree shown in Figure 2. Table S5. ANOVA of the quadratic model for the process parameters optimization of l-asparaginase productivity by Bacillus licheniformis mutant using Box–Behnken central composite design.