MOESM1 of Epistatic interactions between at least three loci determine the “rat-tail” phenotype in cattle

Additional file 1: Figure S1. Phenotype scoring of RTS associated hair conformation and pigmentation traits. Phenotypes and corresponding scores for HS: HS 1: wild type, straight hair, HS 2: hair with fluffy, smooth structure, HS 3: curly hair with a mildly frizzy/wiry structure, HS 4: coarse, frizzy/wiry hair, HS 5: very coarse/wiry hair, HS 6: extremely coarse/wiry HS. Phenotypes and corresponding scores for hair density (HD): HD 1: wild type, full, thick, long hair covering the whole body as well as in the ears and around the eyes, HD 2: scarce hair at the trunk and/or few, short hair in the ears and around the eyes, HD 3: short and sparse hair in the ears and around the eyes as well as thin coat, HD 4: without eye lashes and/or hair in the ears and with very thin hair coat and/or bald areas. Phenotypes and corresponding scores for hair length variations between pigmented and unpigmented areas of spotted animals (HLV): HLV 1: wild type, equally long hair in pigmented and unpigmented sections. HLV 2: pigmented hair mildly shorter than unpigmented hair, HLV 3: pigmented hair moderately shorter than unpigmented hair, HLV 4: pigmented hair extremely shorter than unpigmented hair. Animals without white spotting marks were scored according to their phenotype for RT. Phenotypes and corresponding scores for RTS classification phenotype: RT 1: wild type without any RTS-associated hair conformation malformation, RT 2: mild HLV and/or short hair in ears and short, thin eye lashes, reduced tail switch. RT 3: sparse, coarse hair, moderate HLV, few hairs in ears/around eyes and/or few hair in eye lashes, tail switch only rudimentary. RT 4: very sparse coat, extreme HLV, scarce/wiry hair in pigmented areas, no hair in ears, barely/no eyelashes, extremely visible skin folds at face/throat, bald areas surrounding the eyes/in the ears, missing tail switch. Status of tail switch was only informative, if the end of the tail was pigmented. Phenotypes and corresponding scores for coat colour dilution (Dilu) in pigmented coat sections on a eumelanic (black, brown) and pheomelanic (red, yellow) colour background. Dilu 1: undiluted coat colour, black or red-brown; Dilu 2: mild colour dilution with dark grey colour for the black/brown background and orange/caramel for the red background, Dilu 3: mouse grey/bright grey (black background) or yellow/beige (red background), Dilu 4: extremely diluted, with crème and nearly white pigmentation.