MOESM1 of Direct reprogramming of mouse fibroblasts into neural cells via Porphyra yezoensis polysaccharide based high efficient gene co-delivery

Additional file 1: Figure S1. Fourier-Transform infrared spectra of Porphyra yezoensis polysaccharide (PYP) and ethylenediamine modified Porphyra yezoensis polysaccharide (Ed-PYP). Figure S2. Electrophoretic mobility of plasmid in Ed-PYP-plasmid Ascl1, Brn4 and Tcf3 (pABT) nanoparticles at various weight ratios. Lane 1–3, free plasmids Ascl1, Brn4 and Tcf3 from left to right; Lane 4–9, Ed-PYP: pABT weight ratios of 10:1, 20:1, 40:1, 80:1, 150:1, and 300:1, respectively. Figure S3. Cytotoxicity assay. Bar 1, 3T6 cells treated with Ed-PYP; bar2, free plasmid group Ascl1, Brn4 and Tcf3 (pABT) (control group); bars 3–8, ethylenediamine modified Porphyra yezoensis polysaccharide (Ed-PYP)–pABT nanoparticles at ratios of 20:1, 40:1, 80:1, 150:1, 300:1, and 400:1 from left to right, respectively; bar 9, PEI–pABT; bars 10, Lipofectamine 2000(Lip2000)-pABT. Figure S4. Characterization of ethylenediamine modified Porphyra yezoensis polysaccharide (Ed-PYP)-pABT nanoparticles. (A) Zeta-potential results. Notes: Bar 1, naked plasmid group Ascl1, Brn4 and Tcf3 (pABT); bar 2, Porphyra yezoensis polysaccharide (PYP); bar 3, ethylenediamine modified Porphyra yezoensis polysaccharide (Ed-PYP); bars 4–6, Ed-PYP–pABT nanoparticles prepared at various Ed-PYP: pABT weight ratios-20:1, 40:1, and 80:1 from left to right, respectively (means±standard deviation of measurements from three replicates). (B) Size distribution of the nanoparticles at Ed-PYP: pABT weight ratios of 20:1, 40:1, and 80:1, respectively. (C) Transmission electron microscopy image of the nanoparticles at an Ed-PYP: pABT weight ratio of 40:1. (D) Particle-size distribution of the nanoparticles at an Ed-PYP: pABT weight ratio of 40:1.