MOESM1 of Differential transcriptome analysis of the disease tolerant Madagascar–Malaysia crossbred black tiger shrimp, Penaeus monodon hepatopancreas in response to acute hepatopancreatic necrosis disease (AHPND) infection: inference on immune gene response and interaction

Additional file 1. Transcriptomic analysis (RNA-Seq) details for AHPND-infected P. monodon hepatopancreas samples under APm-CTL, APm-T3, APm-T6, and APm-T24. Table S1: Summary of sequencing reads after filtering. Table S2: Transcriptome de novo assembly Unigenes quality metrics. Figure S1: Distribution of Unigenes based on FPKM values. Figure S2: Distribution of FPKM and TPM values based on length of Unigenes. Table S3: Overview of functional annotation results. Figure S3: Species distribution of transcriptome Unigenes annotated to NR annotation. Figure S4: Functional distribution of Unigenes based on COG analysis. Figure S5: Functional distribution of Unigenes based on GO annotation. Figure S6: Functional distribution of Unigenes based on KEGG annotation. Figure S7: Functionally enriched KEGG pathways based on DEGs. Table S4. Primer sequences designed for qRT-PCR analysis. Figure S8: Relative log2 fold change of P. monodon hepatopancreas C-type lectin gene expression across post-AHPND infection time points. Table S5: One-Way Analysis of Variance (One-Way ANOVA) and post hoc Duncan test showing statistical significance of P. monodon post-AHPND infection C-type Lectin relative gene expressions obtained. Figure S9: Relative log2 fold change of P. monodon hepatopancreas IMD gene expression across post-AHPND infection time points. Table S6: One-Way Analysis of Variance (One-Way ANOVA) and post hoc Duncan test showing statistical significance of P. monodon post-AHPND infection IMD relative gene expressions obtained. Figure S10: Relative log2 fold change of P. monodon hepatopancreas ALF lectin gene expression across post-AHPND infection time points. Table S7: One-Way Analysis of Variance (One-Way ANOVA) and post hoc Duncan test showing statistical significance of P. monodon post-AHPND infection ALF relative gene expressions obtained. Figure S11: Relative log2 fold change of P. monodon hepatopancreas HMGB1 lectin gene expression across post-AHPND infection time points. Table S8: One-Way Analysis of Variance (One-Way ANOVA) and post hoc Duncan test showing statistical significance of P. monodon post-AHPND infection HMGB1 relative gene expressions obtained.