MOESM1 of Development of indirect enzyme-linked immunosorbent assay for detection of porcine epidemic diarrhea virus specific antibodies (IgG) in serum of naturally infected pigs

Additional file 1: Figure S1. Evaluation for working antigen concentration of PEDV. For optimization of working PEDV antigen concentration, 3 NT positive sera (1: 128, 1:64, 1:8 of NT titer) and 3 NT negative sera (1:< 2 of NT titer) were tested at 1:100, 1:200, 1:400 and 1:800 dilutions of PEDV antigen coated ELISA plate. The serum samples were diluted at 1:1000 in this experiment. The highest OD value in NT positive sera and the lowest OD value in NT negative sera were observed for 1:100 dilution of PEDV antigen. Therefore, the working PEDV antigen concentration for the indirect ELISA was set as 1:100 dilution in this study.