MOESM1 of Detecting N-myristoylation and S-acylation of host and pathogen proteins in plants using click chemistry

Additional file 1: Figure S1. Azide fatty acid analogs, but not alkyne fatty acid analogs, interfere with cellular functions. (A) Arabidopsis protoplasts were transformed with FLAG epitope-tagged YFP and treated with different concentrations of the azide fatty acid analog Az12. Cells were incubated overnight and total protein extracted. Anti-FLAG western blotting was used to detect YFP accumulation. Coomassie brilliant blue (CBB) stain was used to visualize total protein and demonstrate equal loading. NT, not transformed. Black dividing lines indicate removal of irrelevant lanes from the blot and gel images. (B) Arabidopsis protoplasts were transformed with FLAG epitope-tagged YFP and treated with different concentrations of the alkyne fatty acid analog Alk12. Cells were incubated overnight and total protein extracted. Anti-FLAG western blotting was used to detect YFP accumulation. CBB stain was used to visualize total protein and demonstrate equal loading.