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MOESM1 of Comparison of four glycosyl residue composition methods for effectiveness in detecting sugars from cell walls of dicot and grass tissues

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posted on 2017-07-14, 05:00 authored by Ajaya Biswal, Li Tan, Melani Atmodjo, Jaclyn DeMartini, Ivana Gelineo-Albersheim, Kimberly Hunt, Ian Black, Sushree Mohanty, David Ryno, Charles Wyman, Debra Mohnen
Additional file 1. Gas chromatographic (GC) profile of the derivatized sugar standards in the alditol acetate (AA) method. The standard mixture consists of 0.5 μg of each sugar (in bold), supplemented with myo-inositol (0.2 μg, in bold) as an internal standard. Note that ribose (in brackets) is also included in the chromatogram shown. Derivatized sugars are separated on a SP-2330 Supelco column (30 m × 0.25 mm, 0.25 μm film thickness) connected to a Hewlett–Packard chromatograph (5890) using helium as the carrier gas with an oven temperature program as described in the “Methods”.

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U.S. Department of Energy

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