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MOESM1 of Citrus limetta peels: a promising substrate for the production of multienzyme preparation from a yeast consortium

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posted on 2019-11-20, 05:01 authored by Maria Shariq, Muhammad Sohail
Additional file 1: Table S1. Regression coefficient and P-values of endoglucanase (EG), β-glucosidase (BGL), xylanase (Xyl), pectinase (PEC) and amylase (AMY) for acid pretreated Citrus lametta peels’ fermentation. Table S2. Regression coefficient and P-values of endoglucanase (EG), β-glucosidase (BGL), xylanase (Xyl), pectinase (PEC) and amylase (AMY) for Plackett-Burman of SSF of alkali pretreated substrate. Table S3. Summary of two-way ANOVA. PBD designs executed for fermentation of acid and alkali pretreated Citrus limetta peels were compared to determine suitability of the substrates. Table S4. Analysis of variance (ANOVA) of (a) EG, (b) BGL, (c) Xyl, and (d) AMY production for regression model. Table S5. Optimum conditions for the production of mulienzyme preparation as suggested by the Box Behnken deisgn. The model also predicted values of individual enzyme activities that can be obtained under optimum conditions. An experiment was conducted under optimum conditions and actual values of the enzyme activities are given. Table S6. Analysis of variance (ANOVA) of pectinase (PEC) response for regression model. Table S7. Rate of hydrolysis of various substrates when loaded with multienzyme preparation from the yeast consortium. Fig. S1. Surface plots of EG showing relationship between (a) Moistening agentand pretreatment. (b) Media pH and pretreatment. (c) Incubation time and pretreatment. (d) Moistening agent and media pH. (e) Moistening agent and incubation time. (f) Media pH and incubation time. Fig. S2. Surface plots of BGL showing relationship between. (a) Moistening agent and pretreatment. (b) Media pH and pretreatment. (c) Incubation time and pretreatment. (d) Moistening agent and media pH. (e) Moistening agent and incubation time. (f) Media pH and incubation time. Fig. S3. Surface plots of XYL showing relationship between (a) Moistening agent and pretreatment. (b) Media pH and pretreatment. (c) Incubation time and pretreatment. (d) Moistening agent and media pH. (e) Moistening agent and incubation time. (f) Media pH and incubation time. Fig. S4. Surface plots of AMY showing relationship between (a) Moistening agent and pretreatment. (b) Media pH and pretreatment. (c) Incubation time and pretreatment. (d) Moistening agent and media pH. (e) Moistening agent and incubation time. (f) Media pH and incubation time. Fig. S5. Saccharification of apple peels, sugarcane bagasse, banana peels, mosambi peels, mango peels, orange peels, pomegranate peels, CMC, Xylan, Pectin by multienzyme preparation.

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Third World Academy of Sciences

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