MOESM1 of Circulating microRNA-21 is an early predictor of ROS-mediated damage in subjects with high risk of developing diabetes and in drug-naïve T2D

Additional file 1. Absorbance (Abs) of plasma samples read at 375 nm and 414 nm wavelength using a spectrophotometer [26, 27]. The ratio between absorbance at 414 nm and 375 nm was calculated. The ratio major than 1.4 was considered sample hemolyzed [26]. Further, we read at 541 nm and 576 nm in order to verify the high levels of hemolysis [26, 27].