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MOESM1 of Biomimetic strategy for constructing Clostridium thermocellum cellulosomal operons in Bacillus subtilis

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posted on 2018-06-07, 05:00 authored by Jui-Jen Chang, Marimuthu Anandharaj, Cheng-Yu Ho, Kenji Tsuge, Tsung-Yu Tsai, Huei-Mien Ke, Yu-Ju Lin, Minh Ha Tran, Wen-Hsiung Li, Chieh-Chen Huang
Additional file 1: Table S1. Details of C. thermocellum genes used in this study. The cellulosomal genes were amplified from C. thermocellum ATCC 27405 genomic DNA using gene-specific primers. Table S2. Primers used for the amplification of specific genes from genomic DNA. Table S3. Sequencing and qPCR primers used for checking the specific genes. Figure S1. Western blotting analysis of CipA using epitope antibody against CipA. (1: negative control, 2: B subtilis WB800− cipA + sdbA, 3: B subtilis WB800− cipA + sdbA, 4: Marker). Figure S2. MASCOT analysis of protein bands.

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Ministry of Science and Technology, Taiwan

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